Michèle Bouchard

Les travaux de recherche de mon équipe visent à mieux évaluer les risques toxicologiques chez l’humain associés à des expositions environnementales et professionnelles à des produits chimiques par l’utilisation de la biosurveillance et de la modélisation toxicocinétique. Nos activités impliquent le développement de méthodes analytiques pour la quantification de faibles niveaux de biomarqueurs de l’exposition à certains polluants chimiques. Ces méthodes permettent d’étudier le comportement cinétique de ces substances dans l’organisme animal et humain. Les données peuvent alors servir au développement de modèles biomathématiques pour décrire le devenir des contaminants d’intérêt dans l’organisme humain et reconstituer les doses absorbées à partir de mesures de biomarqueurs.

Nos travaux consistent également à utiliser la mesure de biomarqueurs et les outils de modélisation toxicocinétique pour l’évaluation de l’exposition à des contaminants et des risques d’effets sanitaires dans la population générale et dans le milieu de travail. Les contaminants étudiés dans ce contexte incluent les pyréthrines naturelles, les pyréthrinoïdes, les phtalimides, les insecticides organophosphorés, les carbamates, les biphényles polychlorés, les hydrocarbures aromatiques polycycliques, le formaldéhyde, le sélénium, les métaux et métalloïdes, comme le mercure, l’arsenic, incluant les métaux rares.

Michèle Bouchard détient un  Ph.D. en Santé publique - option Toxicologie de l'environnement de l’Université de Montréal. Professeure titulaire au Département de santé environnementale et au travail de l’ESPUM, Michèle Bouchard est également titulaire de la Chaire d'analyse et de gestion des risques toxicologiques ainsi que chercheuse régulière à l’IRSPUM.

• 28-02-2017 Un financement de 10 M$ pour des infrastructures de recherche à l’UdeM
• 12-03-2018 Pour un fonds de recherche indépendant sur les pesticides
• 26-11-2019 Santé humaine et santé animale, même combat!

50- Heredia-Ortiz R, Berthet A., Bouchard M*. Toxicokinetic modeling of folpet fungicide and its ring-biomarkers of exposure in humans. Journal of Applied Toxicology 33(7): 607-617, 2013 (résultats utilisés dans l’évaluation Human biomonitoring data collection from occupational exposure to pesticides, par l’ESFA 2017).  

51- Nadeau, S, Bouchard M, Debia M, DeMarcellis-Warin N, Hallé S, Songmene V, Therrien M.-C, Wilkinson K, Ateme-Nguema B, Dufour G, Dufresne A, Fatisson J, Haddad S, Hadioui M, Kouam J, Morency F, Tardif R, Viens M, Weichenthal S, Viau C, Camus M. ÉquiNanos: Innovative team for nanoparticle risk. Invited Technical and commercialization note, Nanomedicine: Nanotechnology, Biology and Medicine 9(1): 22-24, 2013.

52- El Majidi N, Bouchard M*, Carrier G. Relationship between prenatal exposure to polychlorinated biphenyls and mental and motor development during follow-up of children cohorts: a systematic analysis of published epidemiological studies through a standardization of biomonitoring data. Regulatory Toxicology and Pharmacology 66: 130-146, 2013.

53- Normandin L, Bouchard M*,  Ayotte P,  Blanchet C, Becalski A,  Bonvalot Y, Lapointe C, Phaneuf D, Gagné M, Courteau M. Dietary exposure to acrylamide in adolescents from a Canadian urban center. Food and Chemical Toxicology 57: 75-83, 2013. 

54- Heredia-Ortiz R, Bouchard M*. Understanding the linked kinetics of benzo(a)pyrene and 3-hydroxybenzo(a)pyrene biomarker of exposure using physiologically-based pharmacokinetic modelling in rats. Journal of Pharmacokinetics and Pharmacodynamics 40: 669-682, 2013. 

55- Azandjeme CS, Bouchard M*, Fayomi B, Djrolo F, Houinato D, Delisle H. Growing burden of diabetes in Sub-Saharan Africa : Contribution of pesticides? Current Diabetes Reviews 9: 437-449, 2013. 

56- Brisson B, Ayotte P, Normandin L, Gaudreau E, Bienvenu JF, Fennell TR, Blanchet C, Phaneuf D, Lapointe C, Bonvalot Y, Gagné M, Courteau M, Snyder RW, Bouchard M*. Relation between dietary acrylamide exposure and biomarkers of internal dose in Canadian teenagers. Journal of Exposure Science and Environmental Epidemiology 24(2): 215-221, 2014.

57- Miao Q, Bouchard M, Chen D, Burstyn I, Spinelli JJ, Kristan J. Aronson KJ. Assessing traffic and polycyclic aromatic hydrocarbon exposure in Montreal, Canada. Science of the Total Environment 470–471: 945–953, 2014. 

58- El Majidi N, Bouchard M*, Gaétan Carrier. Systematic analysis of the relationship between standardized biological levels of polychlorinated biphenyls and thyroid function in pregnant women and newborns. Chemosphere 98: 1-17, 2014. 

59- Brochu P, Bouchard M*, Haddad S. Physiological daily inhalation rates for health risk assessment in overweight/obese children, adults and elderly. Risk Analysis 34(3): 567-582, 2014. 

60- Normandin L, Bouchard M*, Levallois P, Ayotte P, Ibanez Y, Kennedy G, Chen L, Le C. Biomarkers of arsenic exposure and effects in a Canadian rural population exposed through ground water consumption. Journal of Exposure Science and Environmental Epidemiology, 24(2): 127-134, 2014. 

61- Côté J, Carrier G, Bonvalot Y, Lapointe C, Woollen B, Fuhr U, Tomalik-Scharte D,  Wachall B, Bouchard M*. A novel toxicokinetic model of cypermethrin and permethrin and their metabolites in humans for dose reconstruction from biomarker data. PLoS One 9(2) :e88517. doi: 10.1371/journal.pone.0088517, eCollection 2014. (Résultats utilisés par Santé Canada dans Proposed Re-evaluation Decision (RED) for Cypermethrin (PRVD2016-18).

62- Heredia-Ortiz R, Maître A, Lafontaine M, Bouchard M*. Use of physiologically-based pharmacokinetic modeling to simulate the profiles of 3-hydroxybenzo(a)pyrene in workers exposed to polycyclic aromatic hydrocarbons. PLoS ONE 9(7):e102570. doi : 10.1371/journal.pone.0102570, eCollection 2014. (Travaux sollicités pour consultation par le « Board on Environmental Studies and Toxicology » du « National Academies of Sciences, Engineering, and Medicine »: http://dels.nas.edu/global/best/IRIS-Experts dans le cadre du « Integrated Risk Information System (IRIS) Public Science Meeting June 29-30, 2016 tert-Butyl Alcohol and Benzo[a]pyrene (BaP) ». 

63- Noisel N, Carrier G, Bouchard M*. Study of selenium intake and disposition in various matrices based on mathematical algorithms derived from pooled biomonitoring data. International Journal of Hygiene and Environmental Health, 217: 796-804, 2014.   

64- Azandjeme CS, Delisle H, Fayomi B, Ayotte P, Djrolo F, Houinato D, Bouchard M*. High serum organochlorine pesticide concentrations in diabetics of a cotton producing area of the Republic of Benin (West Africa). Environment International 69: 1-8, 2014. 

65- Azandjeme CS, Bouchard M, Djrolo F, Houinato D, Delisle H. Serum concentrations of organochlorine pesticides associated with diabetes and obesity in Northern Benin, West Africa: A case-control study. Journal of Environmental and Occupational Science 3 (3): 121-129, 2014. 

66- Moreau M, Ouellet N, Ayotte P, Bouchard M*. Effect of benzo(a)pyrene administration dose on levels of exposure biomarkers, DNA adducts and gene expression. Journal of Toxicology and Environmental Health 78: 1-19, 2015.

67- Moreau M, Bouchard M*. Comparison of the kinetics of various biomarkers of benzo[a]pyrene exposure following different routes of entry in rats. Journal of Applied Toxicology. 35(7) :781-790, 2015.

68- Miao Q, Bouchard M, Chen D, Rosenberg MW, Aronson KJ. Commuting behaviours and exposure to air pollution in Montreal, Canada. Science of the Total Environment 508: 193-198, 2015.

69- Ratelle M, Coté J, Bouchard M*. Toxicokinetics of permethrin biomarkers of exposure in orally exposed volunteers. Toxicology Letters 232(2): 369-375, 2015. (résultats utilisés par Santé Canada dans Proposed Re-evaluation Decision (RED) for Permethrin and its Associated End-Use Products (PRVD2017-18).

70- Ratelle M, Côté J, Bouchard M*. Time profiles and toxicokinetic parameters of key biomarkers of exposure to cypermethrin in orally exposed volunteers compared with previously available kinetic data following permethrin exposure. Journal of Applied Toxicology 35(12): 1586-1593, 2015. (Résultats utilisés par Santé Canada dans Proposed Re-evaluation Decision (RED) for Cypermethrin (PRVD2016-18).

71- Moreau M, Ayotte P, Bouchard M*. Kinetics of diol and hydroxybenzo(a)pyrene metabolites in relation with DNA adduct formation and gene expression in rats. Journal of Toxicology and Environmental Health 78: 725-746, 2015.

72- Feseke Keboya S, St-Laurent J, Anassour-Sidi E, Ayotte P, Bouchard M, Levallois P. Arsenic exposure and type 2 diabetes: results of the Canadian Health Measures Survey (2007-2009). Health Promotion and Chronic Diseases Prevention in Canada 35(4): 1-10, 2015. 

73- Ferland S, Côté J, Ratelle M, Thuot T, Bouchard M*. Detailed time profiles of biomarkers of exposure in workers exposed to permethrin in a corn production farm in Quebec, Canada. Annals of Occupational Hygiene 59(9): 1152-1167, 2015. (résultats utilisés dans l’évaluation Human biomonitoring data collection from occupational exposure to pesticides, par l’ESFA 2017).  

74- Ratelle M, Côté J, Bouchard M*. Time courses and variability of pyrethroid biomarkers of exposure in a group of agricultural workers in Quebec, Canada. International Archives of Occupational and Environmental Health 89(5): 767-783, 2016. (résultats utilisés dans l’évaluation Human biomonitoring data collection from occupational exposure to pesticides, par l’ESFA 2017).  

75- Pujalté I, Dieme D, Haddad S, Serventi AM, Bouchard M*. Toxicokinetics of titanium dioxide (TiO2) nanoparticles after inhalation in rats. Toxicology Letters 265: 77-85, 2017.

76- Aouey B, Derbali M, Chtourou Y, Bouchard M, Fetoui H. Pyrethroid insecticide lambda-cyhalothrin and its metabolites induce liver injury through the activation of oxidative stress and proinflammatory gene expression in rats following acute and subchronic exposure. Environmental Science and Pollution Research 24(6): 5841-5856, 2017.

77- Khemiri R, Côté J, Bouchard M*. Documenting the kinetic time course of lambda-cyhalothrin metabolites in orally exposed volunteers for the interpretation of biomonitoring data. Toxicology Letters 276: 115-121, 2017.

78- Pujalté I, Noël A, Dieme D, Haddad S, Bouchard M*. Comparison of three aerosol generation methods for inhalation nanotoxicology studies using titanium dioxide nanoparticles. Toxics 5(14):1-18, 2017; doi:10.3390/toxics5030014.

79- Côté J, Bouchard M*. Dose reconstruction in workers exposed to permethrin and cypermethrin and determination of biological reference values using a toxicokinetic model. Journal of Exposure Sciences and Environmental Epidemiology 28(6):599-614, 2018.

80- Darney K, Bodin L, Bouchard M, Côté J, Volatier J-L, Desgines V. Aggregate exposure of the adult French population to pyrethroids. Toxicology and Applied Pharmacology, 351: 21-31, 2018. https://doi.org/10.1016/j.taap.2018.05.007.

81- Gargouri B, Bhatia HS, Bouchard M*, Fiebich BL, Fetoui H. Inflammatory and oxidative mechanisms potentiate bifenthrin-induced neurological alterations and anxiety-like behavior in adult rats. Toxicology Letters 294: 73-86, 2018.

82- Gargouri B, Yousif NM, Bouchard M, Fetoui H, Fiebich BL. Inflammatory and cytotoxic effects of bifenthrin in primary microglia and organotypic hippocampal slice cultures. Journal of Neuroinflammation 15(1):159, 2018. doi: 10.1186/s12974-018-1198-1.

83- Valcke M, Bourgault MH, Haddad S, Bouchard M, Gauvin D, Levallois P. Deriving a drinking water guideline for a non-carcinogenic contaminant: the case of manganese. Int J Environ Res Public Health 15(6): 1293, 2018. pii: E1293. doi: 10.3390/ijerph15061293.

84- Gargouri B, Yousif NM, Attaai A, Bouchard M, Chtourou Y, Fiebich BL, Fetoui H. Pyrethroid bifenthrin induces oxidative stress, neuroinflammation, and neuronal damage, associated with cognitive and memory impairment in murine hippocampus. Neurochemistry International 120:121-133, 2018.

85- Khemiri R, Côté J, Bouchard M*. Kinetic time course of lambda-cyhalothrin metabolites after dermal exposure in volunteers. Toxicology Letters 296:132-138, 2018.

86- Ratelle M, Skinner K, Laird M, Majowicz S, Brandow D, Packull McCormick S, Bouchard M, Dieme D, Stark K, Aristizabal Henao JJ, Hanning R, Laird B. Implementation of Human Biomonitoring in the Dehcho Region of the Northwest Territories, Canada (2016-2017). Archives of Public Health 76:73, 2018 (open access).

87- Gayrard V, Lacroix MZ, Grandin F, Collet SH, Mila H, Viguié C, Gély C, Rabozzi B, Bouchard M, Léandri R, Toutain P-L, Picard-Hagen N. Differential oral systemic bioavailability of Bisphenol A and Bisphenol S in pigs. Environmental Health Perspectives, 127(7):077005-1-077005-12, 2019.

88- Caron-Beaudoin E, Bouchard M, Wendling G, Barroso A, Bouchard MF, Ayotte P, Frohlich FL, Verner M-A. Urinary and hair concentrations of trace metals in pregnant women from Northeastern British Columbia, Canada: a pilot study. Journal of ExposureScience and Environmental Epidemiology, 29(5):613-623, 2019. doi: 10.1038/s41370-019-0144-3.

89- Gargouri B, Bouchard M, Wilke Saliba S, Fetoui H, Fiebich BL. Repeated bifenthrin exposure alters hippocampal Nurr-1/AChE and induces depression-like behavior in adult rats. Behavioural Brain Research, 370:111898. doi: 10.1016/j.bbr.2019.04.012,2019.

90- Aouey B, Gargouri B, Chtourou Y, Bouchard M, Fetoui H. Lambda-cyhalothrin exposure alters purine nucleotide hydrolysis and nucleotidase gene expression pattern in platelets and liver of rats. Chemico-Biological Interactions 311:108796, 2019. doi: 10.1016/j.cbi.2019.108796.