Madhu Anand-Srivastava



Titre:

Professeure titulaire
Département de pharmacologie et physiologie
Faculté de médecine
Université de Montréal

Adresse: Pavillon Paul-G.-Desmarais
C. P. 6128,  succursake Centre-ville
Montréal (Québec) H3C 3J7
Local: Bureau 2127
Téléphone: 514 343-2091
Courriel: madhu.anand-srivastava@umontreal.ca


Formation

  • M.Sc. Medical Biochemistry, Delhi University, Delhi, India (1972)
  • Ph.D. Physiology, University of Manitoba, Winnipeg, Manitoba (1978)

Thèmes de recherche

  • G proteins, oxidative stress and cell signaling in cardiovascular system in different pathologies such as hypertension, diabetes.
  • Role of vasoactive peptides and signaling in Gi and Gq protein expression and their role in vascular smooth muscle cell hyperproliferation and hypertrophy in hypertension.
  • Natriuretic peptide receptors, their regulation in cardiovascular physiology and pathology.

Approches techniques

  • Cell culture
  • Westernblotting
  • Gene Knockdown using antisense and siRNA
  • Cell proliferation (DNA synthesis, Thymidine incorporation)
  • Hypertrophy (Protein synthesis, Leucine incorporation)
  • Blood pressure measurement
  • Genetic and experimentally hypertensive rat models

Membres du laboratoire

  • Yuan Li, Research Assitant
  • Sara Almajdoob, Research Assistant
  • Md Ekhtear Hossain, Postdoctoral Fellow
  • Stephanie Polchtchikov, M.Sc student
  • Vanessa Truong, M.Sc student (co-director)

Publications

Hossain E, Sarkar O, Li Y, Anand-Srivastava MBInhibition of overexpression of Giα proteins and nitroxidative stress contribute to sodium nitroprusside-induced attenuation of high blood pressure in SHR. Physiol Rep. 2018 Mar;6(6):e13658. doi: 10.14814/phy2.13658.

Jain A, Anand-Srivastava MB. Natriuretic peptide receptor-C-mediated attenuation of vascular smooth muscle cell hypertrophy involves Gqα/PLCβ1 proteins and ROS-associated signaling. Pharmacol Res Perspect. 2018 Feb;6(1). doi: 10.1002/prp2.375.

Madiraju P, Hossain E, Anand-Srivastava MB. Natriuretic peptide receptor-C activation attenuates angiotensin II-induced enhanced oxidative stress and hyperproliferation of aortic vascular smooth muscle cells. Mol Cell Biochem. 2018 Nov;448(1-2):77-89. doi: 10.1007/s11010-018-3316-x. Epub 2018 Feb 7.

Rahali S, Li Y, Anand-Srivastava MB. Contribution of oxidative stress and growth factor receptor transactivation in natriuretic peptide receptor C-mediated attenuation of hyperproliferation of vascular smooth muscle cells from SHR. PLoS One. 2018 Jan 24;13(1):e0191743. doi: 10.1371/journal.pone.0191743. eCollection 2018.PMID:29364969

Jain A and Anand-Srivastava MB. Natriuretic Peptide Receptor-C-mediated attenuation of Vascular Smooth Muscle Cell hypertrophy involves Gqα/PLCβ1 Proteins and ROS-associated signaling. Pharmacol.Res.Perspect. 2018;e00375. https://doi.org/10.1002/prp2.375

Almajdoob S, Hossain E, Anand-Srivastava MB. Resveratrol attenuates hyperproliferation of vascular smooth muscle cells from spontaneously hypertensive rats: Role of ROS and ROS-mediated cell signaling. Vascul. Pharmacol. 2017 Dec 19. pii: S1537-1891(17)30324-5. doi: 10.1016/j.vph.2017.12.064. [Epub ahead of print]PMID:29277292

Balakumar P, Anand-Srivastava MB, Jagadeesh G. Renin-angiotensin-aldosterone: An inclusive, an invigorative, an interactive and an interminable system. Pharmacol Res. 2017 Nov;125(Pt A):1-3. doi: 10.1016/j.phrs.2017.07.003. Epub 2017 Jul12..PMID:28711403

Hossain E, Anand-Srivastava MB. Resveratrol prevents angiotensin II-induced hypertrophy of vascular smooth muscle cells through the transactivation of growth factor receptors. Can J Physiol Pharmacol. 2017 Aug;95(8):945-953. doi: 10.1139/cjpp-2017-0164. Epub 2017 Jul 13.PMID:28704617

Sarkar O, Li Y, Anand-Srivastava MB. Nitric oxide attenuates overexpression of Giα proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling. PLoS One. 2017 Jul 10;12(7):e0179301. doi: 10.1371/journal.pone.0179301. eCollection 2017.PMID:28692698

Ali El-Basyuni Y, Li Y, Anand-Srivastava MB. Knockdown of Inhibitory Guanine Nucleotide Binding Protein Giα-2 by Antisense Oligodeoxynucleotides Attenuates the Development of Hypertension and Tachycardia in Spontaneously Hypertensive Rats. J Am Heart Assoc. 2016 Oct 26;5(11). pii: e004594.PMID:27912212

Atef ME, Anand-Srivastava MB. Role of PKCδ in Enhanced Expression of Gqα/PLCβ1 Proteins and VSMC Hypertrophy in Spontaneously Hypertensive Rats. PLoS One. 2016 Jul 5;11(7):e0157955. doi: 10.1371/journal.pone.0157955. eCollection 2016.PMID:27379421

Atef ME, Anand-Srivastava MB. Oxidative stress contributes to the enhanced expression of Gqα/PLCβ1 proteins and hypertrophy of VSMC from SHR: role of growth factor receptor transactivation. Am J Physiol Heart Circ Physiol. 2016 Mar 1;310(5):H608-18. doi: 10.1152/ajpheart.00659.2015. Epub 2016 Jan 8.PMID:26747500

Bou Daou G, Li Y, Anand-Srivastava MB. Enhanced expression of Giα proteins contributes to the hyperproliferation of vascular smooth muscle cells from spontaneously hypertensive rats via MAP kinase- and PI3 kinase-independent pathways. Can J Physiol Pharmacol. 2016 Jan;94(1):49-58. doi: 10.1139/cjpp-2015-0146. Epub 2015 Jun 18.PMID:26524499